The Amazon rainforest is one of Earth’s most diverse ecosystems, playing a key role in maintaining regional and global climate stability. However, recent changes in land use, vegetation, and the climate have disrupted biosphere-atmosphere interactions, leading to significant alterations in the water, energy, and carbon cycles. …Here, we quantify the relative contributions of deforestation and global climate change to observed shifts in key Amazonian climate parameters. We analyzed long-term atmospheric and land cover change data across 29 areas in the Brazilian Legal Amazon from 1985 to 2020. …While the rise in atmospheric methane (CH4) and carbon dioxide (CO2) mixing ratios is primarily driven by global emissions, deforestation has significantly increased surface air temperatures and reduced precipitation during the Amazonian dry season. Over the past 35 years, deforestation has accounted for approximately 74% of the ~ 21 mm dry season decline and 16.5% of the 2°C rise in maximum surface air temperature.